Frequency of Human Papilloma Virus Genotypes in Patient Samples From Isfahan (Iran) Using Polymerase Chain Reaction Reverse Dot Blot Method

Detection of human papilloma virus in paraffin-embedded tissue using the polymerase chain reaction

Human papilloma virus (HPV) DNA sequences have been detected in paraffin-embedded tissue using an enzymatic in vitro amplification technique known as the polymerase chain reaction. Amplification of a HPV DNA sequence before its detection with a cDNA probe significantly increases the rapidity as well as the sensitivity of detection such that a single 5-10-micron thick paraffin-embedded tissue se...

Diagnosis of Foot-and-Mouth Disease Virus by Real Time Reverse Transcription Polymerase Chain Reaction Assay in Iran

Background and Aims: Accurate and rapid diagnosis is necessary for effective control and prevention of foot-and-mouth disease (FMD). In present study, was evaluated real time reverse transcription-polymerase chain reaction (rRT-PCR) assay along with diagnostic routine methods for the detection of all seven serotypes of FMD virus (FMDV), namely O, C, A, SAT1, 2, 3 and Asia 1 in biological sample...

Detection of Plasmodium falciparum Directly from Blood Samples Using the Polymerase Chain Reaction

Characterization of Iranian Isolates of Canine Parvovirus in Fecal Samples Using Polymerase Chain Reaction Assay

Despite the widespread prevalence of canine parvovirus disease (CPV) in Iranian dog population,molecular diagnosis of CPV variants, and investigation of the trends of its genetic changes is a new effort. Inthis study 50 samples from dogs suspicious of infection with clinical signs of diarrhea and vomiting, and 25samples from dogs suspected of infection with general symptoms su...

Molecular detection of Ehrlichia spp. in blood samples of dogs in southern Iran using polymerase chain reaction

Ehrlichiosis is a zoonotic disease which has been reported from some regions of Iran. This study was aimed to determine the presence and prevalence of ehrlichiosis in suspected dogs referred to the Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran using polymerase chain reaction(PCR). Blood samples were collected from 98 suspected dogs with at least one of the five following findi...